Effect of tautomerization on the fast-atom bombardment tandem mass spectra of azo dyes

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Fast - atom - bombardment mass spectrometry

A detailed study of the mass spectra of peptides produced by the new technique of fast-atom bombardment is reported. Molecular weights of unmodified peptides containing up to 21 amino acids have been determined. In favourable cases, the molecular-weight determination may be made on as little as 0.1 nmol of sample. Positive-ion and negative-ion spectra are obtained with equal facility. With samp...

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FABMS analysis of T-lb peptide before and after one cycle of Edman degradation indicated an unblocked N-terminal Thr residue for this tryptic peptide. In contrast , our data showed a molecular protonated ion, MH + for T- la peptide at 655 mass units (mu) which is 42 mu higher than the MH ion of T- 1b peptide. In addition, T- la peptide was not amenable to one cycle of manual Edman degrada...

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ژورنال

عنوان ژورنال: Journal of the American Society for Mass Spectrometry

سال: 1994

ISSN: 1044-0305,1879-1123

DOI: 10.1016/1044-0305(94)85056-9